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Composition | capacity | unit |
---|---|---|
CaCl2·2H2O | 0.01 | g |
Ferric ammonium citrate | 5.00 | mg |
Distilled water | 1000.00 | ml |
NH4Cl | 1.00 | g |
MgSO4·7H2O | 0.20 | g |
Na2HPO4·2H2O | 4.50 | g |
KH2PO4 | 1.50 | g |
MnSO4·H2O | 0.01 | g |
Na-Pyruvate | 1.50 | g |
Trace element solution SL-6 (see medium 27) | 3.00 | ml |
** ph : -
** memo : Adjust pH to 7.1. Agar if necessary 15.0 g. Distribute in 30 - 50 ml amounts in Erlenmeyer flasks and sterilize for 15 minutes at 121°C. Incubate without agitation at 65°C.
** additional actions :