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Media No. 626 HETEROTROPHIC MEDIUM H3P

Composition

capacity

unit

Solution E:

Composition	capacity	unit
Na-lactate	1.000	g
Na-pyruvate	1.000	g
D-Mannitol	1.000	g
D-Glucose	2.000	g
Distilled water	50.000	ml/L
pH 7.0

Solution C:

Composition	capacity	unit
Ferric ammonium citrate	0.050	g
Distilled water	20.000	ml

Solution A:

Composition	capacity	unit
Distilled water	50.000	ml
KH₂PO₄	2.300	g
Na₂HPO₄ . 2H₂O	2.900	g

Solution D:

Composition	capacity	unit
Yeast extract	1.000	g
Na-acetate	1.000	g
DL-Malate	1.000	g
Distilled water	30.000	ml
pH 7.0
Na₂-succinate	1.000	g

Solution B:

Composition	capacity	unit
Trace element sol. SL-6 (see medium 232)	5.000	ml
Distilled water	850.000	ml
Agar (if necessary)	15.000	g
NH₄Cl	1.000	g
CaCl₂ . 2H₂O	0.010	g
MgSO₄. 7H₂O	0.500	g
MnCl₂ . 4H₂O	0.005	g
NaVO₃ . H₂O	0.005	g

Standard vitamin solution:

Composition	capacity	unit
Riboflavin	10.000	mg
Nicotinic acid	50.000	mg
Pyridoxine-HCl	50.000	mg
Ca-pantothenate	50.000	mg
Biotin	0.100	mg
Folic acid	0.200	mg
Distilled water	100.000	ml
Thiamine-HCl . 2H₂O	50.000	mg
Vitamin B₁₂	1.000	mg

** ph : 7.0

** memo : H3P is a heterotrophic medium for growth, purity checking and isolation of a broad spectrum of
aerobic bacteria (Ref. 3367).
Solutions A, B, C and D are autoclaved separately for 15 min at 121°C, cooled down to 50°C and then
mixed aseptically with filter-sterilized solution E (at 50°C) and 5.0 ml of standard vitamin
solution. The final pH of this medium should be 6.8 without adjustment

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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