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Media No. 650 PYGV AGAR

Composition

capacity

unit

Agar (Bacto)

15.00

Sterilize 20 min./121°C. After cooling to 60°C add to the medium:

Composition	capacity	unit
Glucose sol. (2.5%, sterile-filtered)	10.00	ml
Vitamin sol. (double conc.)	5.00	ml

Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l medium of 6 N KOH).

Mineral salt sol. ("Hutner/Cohen-Bazire")

20.00

Composition	capacity	unit
Nitrilotriacetic acid (NTA)	10.00	g
Metall salt sol. "44" (see medium 638)	50.00	ml
Distilled water	900.00	ml
Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2 SO4. Adjust volume to 1000.0 ml with distilled water.
MgSO₄ . 7H₂O	29.70	g
CaCl₂ . 2H₂O	3.34	g
Na₂MoO₄ . 2H₂O	12.67	mg
FeSO₄ . 7H₂O	99.00	mg

Yeast extract (Bacto)

0.25

Distilled water

965.00

Vitamin solution (double conc.):

5.00

Composition	capacity	unit
Biotin	4.00	mg
Folic acid	4.00	mg
Pyridoxine-HCl	20.00	mg
Riboflavine	10.00	mg
Nicotinamide	10.00	mg
D-Ca-pantothenate	10.00	mg
p-Aminobenzoic acid	10.00	mg
Distilled water	1000.00	ml
Store in the dark and cold (5°C)
Thiamine-HCl . 2H₂O	10.00	mg
Vitamin B₁₂	0.20	mg

Peptone (Bacto)

0.25

** ph : 7.2

** memo : -

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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