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Media No. 651 SULFITOBACTER PONTIACUS MEDIUM
| Composition | capacity | unit |
|---|---|---|
| Peptone | 0.50 | g |
| Yeast extract | 1.00 | g |
| HEPES | 8.00 | g |
| Na-acetate | 20.00 | mM |
| MgSO4·7H2O | 1.00 | g |
| Trace element solution (see medium 539) | 1.00 | ml |
| K2HPO4 | 1.00 | g |
| CaCl2·2H2O | 0.05 | g |
| NaCl | 15.00 | g |
| NH4Cl | 0.50 | g |
| Biotin | 0.10 | g |
| Distilled water | 1000.00 | ml |
** ph : 7.5-7.8
** memo : Prepare the HEPES, NaCl, K_2_HPO_4_ and NH_4_Cl as a basal salt solution, adjusting the pH to 7.5-7.8 with NaOH before sterilisation. All other components are added from sterile stock solutions.
** additional actions :