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Media No. 683 Nitrate mineral salts medium (NMS)

Composition

capacity

unit

MgSO₄·7H₂O

1.0

CaCl₂·6H₂O

0.20

KNO₃

1.0

Chelated Iron Solution (see below)

2.0

Composition	capacity	unit
Ferric (III) ammonium citrate·	0.1	g
EDTA, sodium salt.	0.2	g
HCl (concentrated)	0.3	ml
Distilled deionized water	100.0	ml
·0.5 g of Ferric (III) chloride may be substituted. Use 2.0 ml of this chelated iron solution per liter of final medium.

Trace Element Solution (see below)

0.5

Composition	capacity	unit
EDTA	500.0	mg
Distilled water	1.0	L
Autoclave at 121C for 15 minutes.
FeSO₄ . 7H₂O	200.0	mg
ZnSO₄ . 7H₂O	10.0	mg
MnCl₂ . 4H₂O	3.0	mg
H₃BO₃	30.0	mg
CoCl₂ . 6H₂O	20.0	mg
CaCl₂ . 2H₂O	1.0	mg
NiCl₂ . 6H₂O	2.0	mg
Na₂MoO₄ . 2H₂O	3.0	mg

Purified Agar (e.g., Oxoid L28)

12.5

Distilled deionized water

1.0

KH₂PO₄

0.272

Na₂HPO₄·12H₂O

0.717

Adjust pH to 6.8. Autoclave at 121C for 15 minutes.

** ph : 6.8

** memo : -

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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