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Media No. 684 Modified chopped meat medium

Composition

capacity

unit

Ground beef (fat-free)

500.0

Distilled water

1.0

Vitamin K₁ Solution:

Composition	capacity	unit
95% Ethanol	30.0	ml
Store solution in brown bottle under refrigeration. Discard after one month.
Vitamin K₁	0.15	ml

To this filtrate, add:

Composition	capacity	unit
Trypticase Peptone (BD 211921)	30.0	g
Yeast extract	5.0	g
0.025% Resazurin	4.0	ml
Agar (if necessary)	20.0	g
L-Cysteine . HCl	0.5	g
Hemin Solution (see below)	10.0	ml
Adjust medium for final pH 7.0. Anaerobically dispense 7 ml into tubes containing meat particles (1 part meat to 5 parts fluid) under the same gas phase.
K₂HPO₄	5.0	g
Boil and cool medium under 80% N₂, 10% H₂, 10% CO₂, and add:
Vitamin K₁ Solution (see below)	0.2	ml

1N NaOH

25.0

Mix meat, water and NaOH and bring to a boil with stirring. Cool to room temperature, skim fat from surface, and filter, retaining both meat particles and filtrate. To filtrate, add sufficient distilled water to restore volume to 1.0 L.

Hemin Solution:

Composition	capacity	unit
Hemin	50.0	mg
N NaOH	1.0	ml
Distilled water	100.0	ml
Autoclave at 121C for 15 minutes.

** ph : -

** memo : -

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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