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| Composition | capacity | unit |
|---|---|---|
| MgSO4·7H2O | 0.5 | g |
| Polypepton· | 10 | g |
| Yeast extract | 2 | g |
| Distilled water | 250 | ml |
| Seawater·· | 750 | ml |
| Agar (if needed) | 15 | g |
** ph : -
** memo : pH 7.2 - 7.4.
*Wako Pure Chemical Ind., Ltd., Osaka, Japan.
**Filtered, aged seawater or artificial seawater (Daigo"s Artificial Seawater SP*)
** additional actions :