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Media No. 726 CLOSTRIDIUM CK MEDIUM
| Composition | capacity | unit |
|---|---|---|
| Trypticase Soy Broth (BBL no. 11774) | 5.5 | g |
| Distilled water | 1.0 | L |
| Dissolve dehydrated TSB medium and resazurin, bring to the boil and cool to room temperature while sparging with 100% N2 gas. Adjust pH to 5.5, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose from a separately sterilized anoxic stock solution. | ||
| Resazurin (0.1% w/v) | 0.5 | ml |
| D-Glucose | 2.0 | g |
** ph : -
** memo : Prepare TSB anaerobically under 100% N_2_. Adjust pH to 5.5 prior to dispensing and autoclaving. Add glucose from separately sterilized, anaerobic 20% (w/v) solution.
** additional actions :