JOIN US
KOR
Korean Collection for Type Cultures
KCTC provides the distribution services
Find Resources
Media Information
Media No. 768 CLOSTRIDIUM CHARTATABIDUM MEDIUM
| Composition | capacity | unit | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Yeast extract(OXOID) | 0.50 | g | |||||||||||||||||||||
| Na-resazurin solution (0.1% w/v) | 0.5 | ml | |||||||||||||||||||||
| K2HPO4 | 0.30 | g | |||||||||||||||||||||
| L-Cysteine-HCl·H2O | 0.25 | g | |||||||||||||||||||||
| Mineral solution (see below) | 38.00 | ml | |||||||||||||||||||||
|
|||||||||||||||||||||||
| Clarified rumen fluid | 200 | ml | |||||||||||||||||||||
| Trypticase peptone (BBL) | 2.00 | g | |||||||||||||||||||||
| Na2CO3 | 4.0 | g | |||||||||||||||||||||
| Haemin solution(0.05% w/v, see medium 704) | 2.0 | ml | |||||||||||||||||||||
|
|||||||||||||||||||||||
| Na2S·9H2O | 0.25 | g | |||||||||||||||||||||
| Distilled water | 760 | ml | |||||||||||||||||||||
| Cellobiose | 5.0 | g | |||||||||||||||||||||
| Dissolve ingredients (except carbonate, cellobiose, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 100% CO2 gas atmosphere. Add the carbonate and equilibrate the medium with the CO2 gas to pH 6.8. Distribute under CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cellobiose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N 2 gas. Cellobiose has to be sterilized by filtration. Adjust pH of complete medium to 6.7 - 6.8, if necessary. | |||||||||||||||||||||||
** ph : -
** memo : -
** additional actions :