Korean Collection for Type Cultures

KCTC provides the distribution services

Find Resources

Media Information

Media No. 777 MMB medium

Composition

capacity

unit

Peptone

0.1

Yeast extract

0.1

(NH₄)₂SO₄

0.2

Glucose

1.0

Trace Vitamin (see below)

10.0

Composition	capacity	unit
Biotin	2.0	mg
Folic acid	2.0	mg
Riboflavin	5.0	mg
Nicotinic acid	5.0	mg
Calcium pantothenate	5.0	mg
p-Aminobenzoic acid	5.0	mg
Lipoic acid	5.0	mg
Distilled water	1.0	L
Vitamin B₁₂	0.1	mg
Pyridoxine . HCl	10.0	mg
Thiamine . HCl	5.0	mg

Adjust pH to 7.0 - 7.5. For solid medium, add 15.0 g/L agar.

Hunter's basal salts (see below)

20.0

Composition	capacity	unit
Nitrilotriacetic acid	10.0	g
Nicotinic acid	50.0	mg
Biotin	0.5	mg
Metals ''44'' (see below)	50.0	ml
Distilled water	950.0	ml
Dissolve and neutralized nitrilotriacetic acid in 500.0 ml distilled water with the addition of KOH. Add the remaining chemicals in order listed. Adjust pH to 6.8, and bring volume to 1.0 L with distilled water.
MgSO₄ . 7H₂O	14.45	g
CaCl₂ . 2H₂O	4.42	g
(NH₄)₆Mo₇O₂₄ . 4H₂O	9.25	mg
FeSO₄ . 7H₂O	99.0	mg
Thiamine . HCl	25.0	mg

Distilled water

1.0

Metals ``44''

Composition	capacity	unit
Distilled water	100	ml
Na₂ . EDTA	250.0	mg
ZnSO₄ . 7H₂O	1095.0	mg
FeSO₄ . 7H₂O	500.0	mg
MnSO₄ . xH₂O	154.0	mg
CuSO₄ . 5H₂O	39.2	mg
Co(NO₃)₂ . 6H₂O	24.8	mg
Na₂B₄O₇ . 10H₂O	17.7	mg

** ph : -

** memo : -

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

Copyright 2019 KCTC. All rights reserved.