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Media Information
Media No. 788 HESP1/SR1/TMC4/LUP MEDIUM
| Composition | capacity | unit | ||||||
|---|---|---|---|---|---|---|---|---|
| CuCl2·2H2O | 2.00 | mg | ||||||
| NH4Cl | 1.0 | g | ||||||
| K2HPO4 | 0.3 | g | ||||||
| KCl | 0.1 | g | ||||||
| NaCl | 0.6 | g | ||||||
| MgCl2·6H2O | 0.2 | g | ||||||
| KH2PO4 | 0.3 | g | ||||||
| HCl (25%; 7.7 M) | 10.00 | ml | ||||||
| FeCl2·4H2O | 1.50 | g | ||||||
| CaCl2·2H2O | 0.1 | g | ||||||
| CoCl2·6H2O | 190.00 | mg | ||||||
| ZnCl2 | 70.00 | mg | ||||||
| Yeast extract | 1.0 | g | ||||||
| MnCl2·4H2O | 100.00 | mg | ||||||
| Trace element solution SL-10 | 1.5 | ml | ||||||
| H3BO3 | 6.00 | mg | ||||||
| NiCl2·6H2O | 24.00 | mg | ||||||
| Na2MoO4·2H2O | 36.00 | mg | ||||||
| Distilled water | 990.00 | ml | ||||||
| First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml. | ||||||||
| Resazurin | 0.5 | mg | ||||||
| Prepare medium anaerobically under 80% N2 + CO2 gas mixture. After dissolving the ingredients, adjust pH to 7.0. Heat medium to boiling, cool to room temperature under N2 + CO2 gas and dispense under same gas in anaerobe tubes. After autoclaving add per 10 ml medium from anaerobic, sterile stock solutions: NaHCO3, 10% w/v 0.40-0.80 ml | ||||||||
| Substrate solution (see below) | 0.20 | ml | ||||||
|
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| The final pH of the completed medium should be around 7.0 (for KCTC 5599-5560 ) | ||||||||
| Cysteine-HCl·H2O | 0.5 | g | ||||||
| Distilled water | 1000.0 | ml | ||||||
| Na2S·9H2O, 3% w/v | 0.10 | ml | ||||||
** ph : -
** memo : -
** additional actions :