Korean Collection for Type Cultures

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Media No. 805 SPOROLITUUS MEDIUM

Composition

capacity

unit

K₂HPO₄

1.5

MgCl₂

0.2

NaCl

0.9

FeSO4 x 7 H2O solution (0.1% w/v)

0.5

Composition	capacity	unit
FeSO4 x 7 H2O	1.0	g
H2SO4 (0.1 N)	1000	ml

Modified Wolin's mineral solution (see medium no.918)

Sodium resazurin (0.1% w/v)

0.5

Tryptone (BD Bacto)

2.0

KH₂PO₄

0.75

Yeast Extract (OXOID)

2.0

NH₄Cl

0.9

Sodium citrate

2.0

Na₂S·9H₂O

0.3

Wolin's Vitamin Solution

5.0

Composition	capacity	unit
biotin	2.0	mg
Folic acid	2.0	mg
pyridoxine-HCl	10.0	mg
Thiamine	5.0	mg
Riboflavin	5.0	mg
Nicotinic acid	5.0	mg
pantothenic acid	5.0	mg
Vitamin B₁₂	0.1	mg
Para-amino benzoic acid	5.0	mg
(DL)-alpha-Lipoic acid	5.0	mg
dH₂O	1.0	L
The solution is filter-sterilised and stored at 4C

Dissolve ingredients (except ferrous sulfate, citrate, vitamins and sulfide), adjust pH to 6.5 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add ferrous sulfate and dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add citrate, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and adjust pH of complete medium to 7.0, if necessary.

Distilled water

1.0

** ph : -

** memo : -

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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