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Media No. 806 CLOSTRIDIUM VINCENTII MEDIUM

Composition

capacity

unit

Trypticase

0.4

Boil, cool to room temperature while flushing the medium with 80% N₂ + 20% CO₂ gas mixture. Dispense in anaerobic vessels under same gas and autoclave. Complete medium by adding from following sterile, anaerobic stock solutions:

Composition	capacity	unit
pH of the completed medium is 6.5.
NaHCO₃, 5% (w/v) (N₂ + CO₂) 0.2 ml/10 ml
Lactose, 10% (w/v) (N₂) 0.2 ml/10 ml
Cysteine-HCl . H₂O, 3% (w/v) (N₂) 0.1 ml/10 ml
Na₂S . 9H₂O, 3% (w/v) (N₂) 0.1 ml/10 ml
Na-phosphate buffer, 0.2M, pH 6.5 (N₂) 0.2 ml/10 ml

Yeast extract

1.0

Distilled water

700.0

Resazurin

0.5

Sea water, natural

300.0

NH₄NO₃

0.1

Vitamin solution (see below)

10.0

Composition	capacity	unit
Biotin	2.000	mg
Folic acid	2.000	mg
Pyridoxine-HCl	10.000	mg
Riboflavin	5.000	mg
Nicotinic acid	5.000	mg
D-Ca-pantothenate	5.000	mg
p-Aminobenzoic acid	5.000	mg
Lipoic acid	5.000	mg
Distilled water	1000.000	ml
Thiamine-HCl . 2H₂O	5.000	mg
Vitamin B₁₂	0.100	mg

** ph : -

** memo : -

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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