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Media Information
Media No. 808 Medium for strain Red1
| Composition | capacity | unit |
|---|---|---|
| MgCl3·6H2O | 4.5 | g |
| NH4Cl | 1 | g |
| NaCl | 17 | g |
| CaCl2·2H2O | 0.375 | g |
| Yeast extract | 3 | g |
| K2HPO4·3H2O | 0.08 | g |
| NaNO3 | 2.55 | g |
| Sodium acetate | 0.82 | g |
| NaHCO3 | 3.6 | g |
| Distilled water | 1000 | ml |
** ph : -
** memo : Dissolve ingredients (except yeast extract and sodium acetate) and adjust the pH to 7.0-7.2. Prepare medium anaerobically under 80% N_2_ and 20% CO_2_ gas mixture. After autoclaving, add yeast extract and acetate from sterile stock solutions prepared under N_2_ atmosphere. Sodium nitrate can be substituted by feffic citrate (20mM)
** additional actions :