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Media Information
Media No. 863 HOWARDELLA MEDIUM
| Composition | capacity | unit |
|---|---|---|
| MgCl2·6H2O | 1.10 | g |
| Distilled water | 1000.00 | ml |
| Urea | 1.00 | g |
| Resazurin | 0.50 | mg |
| Bacto Casitone (DIFCO) | 20.00 | g |
| Yeast extract | 5.00 | g |
| Na2HPO4 | 5.00 | g |
| Na-thioglycolate | 0.75 | g |
** ph : -
** memo : Dissolve ingredients (except urea and thioglycolate), boil medium for 1 min., then cool to room temperature under 80% N_2_ and 20% CO_2_ gas mixture.
Add thioglycolate, dispense the medium under same gas atmosphere in culture vessels and autoclave.
After autoclaving add urea from a 10% (w/v) stock solution sterilized by filtration and stored under N_2_. Adjust pH of completed medium to 7.4.
** additional actions :