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Media Information
Media No. 9 Escherichia Medium
| Composition | capacity | unit |
|---|---|---|
| Casein Hydrolysate(NBCo) | 6.0 | g |
| K2HPO_4 _ | 0.2 | g |
| FeSO_4 _·7H2O | 5.0 | mg |
| Add distilled water to 700 ml. Dissolve constituents with gentle heating in the order shown above, using a few drops of HCl to dissolve the asparagine; adjust pH to 7.2. Boil and filter. After cooling, add 2.0 g of glycerol and 12.5 g of agar. Make up volume with distilled water to 1 L and autoclave to dissolve the agar. Readjust pH to 7.2 and add 400 `mug `of crystallized vitamin B12· After mixing, dispense in 10.0 ml amounts. Autoclave at 15 lbs. for 15 minutes. Allow to set in a slanted position. | ||
| L-asparagine | 0.15 | g |
| MgSO4·7H2O | 0.2 | g |
** ph : 7.2
** memo : -
** additional actions :