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Media No. 928 MMJHS medium
| Composition | capacity | unit | ||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NaNO3 | 1.0 | g | ||||||||||||||||||||||||||||||||||||
| Balch's vitamin sol. (see medium 1335) | 10.0 | ml | ||||||||||||||||||||||||||||||||||||
| NaHCO3 | 1.0 | g | ||||||||||||||||||||||||||||||||||||
| Sulfur (powder) | 3.0 | g | ||||||||||||||||||||||||||||||||||||
| Na2S2O3·5H2O | 1.0 | g | ||||||||||||||||||||||||||||||||||||
| MJ synthetic seawater | 1.0 | L | ||||||||||||||||||||||||||||||||||||
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| Mix ingredients except sulfur, adjust pH to 6.5 - 7.0 with NaOH and filter-sterilize. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) containing sulfur under a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Pressurize the inoculated bottles to 300 kPa H2-CO2 (4:1, v/v). | ||||||||||||||||||||||||||||||||||||||
** ph : -
** memo : -
** additional actions :