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Media Information
Media No. 941 KPL MEDIUM
| Composition | capacity | unit |
|---|---|---|
| White table wine (see below) | 140 | ml |
| Agar | 15 | g |
| Glucose | 10 | g |
| Lactic acid whey (see below) | 1000 | ml |
| Galactose | 10 | g |
| Tween 80 | 1.0 | ml |
| Adjust pH to 5.5 | ||
| White table wine: Filter-sterilize and add to autoclaved basal medium | ||
| Lactic acid whey: Adjust 10% skim milk to pH 5.5 with lactic acid and heat at 100°C for 30 minutes. Remove precipitate by filtration. For preparing broth medium, adjust filtrate to pH 7.0 with 2 N NaOH and heat at 100°C for 30 minutes. Remove precipitate by filtration. Adjust filtrate to pH 5.5 with 2 N HCl. |
** ph : -
** memo : -
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