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Media No. 95 Beef Liver Medium for Anaerobes
| Composition | capacity | unit | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Beef liver (cut up in small pieces) | 500.0 | g | |||||||||
| Tap water | 1.0 | L | |||||||||
| Soak beef liver overnight in refrigerator. Skim fat off top. Autoclave for 10 minutes at 15 lbs. Filter through cheesecloth. Save meat. Add: | |||||||||||
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** ph : 8.0
** memo :
Adjust pH to 8.0. Filter through paper and make up volume to 1 L with more tap water. Place a small amount of CaCO_3_
in each tube; add meat to depth of 1/2 inch; cover with broth to a total depth of 2 inches.
** additional actions :