Korean Collection for Type Cultures

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Media Information

Media No. 960 MODIFIED NOS MEDIUM

Composition

capacity

unit

Yeast extract (BBL)

0.25

L-Cysteine·HCl·H₂O

0.1

Sodium thioglycolate

0.05

Heart infusion broth (Difco)

1.25

0.1% Resazurin solution

0.1

Distilled water

7.5% NaHCO3 solution

2.67

Trypticase peptone (BBL)

1.0

VFA solution (see below)

Composition	capacity	unit
Isobutyric acid	0.5	ml
Valeric acid	0.5	ml
2-Methylbutyric acid	0.5	ml
Isovaleric acid	0.5	ml
0.1 N NaOH	100	ml
Add volatile fatty acids to about 70 - 80 ml of NaOH solution and mix. Add NaOH solution to bring final volume to 100 ml. If not used the same day, filter-sterilize and store at 4C

Combine the above ingredients and bring to a boil in a round bottom flask while flushing with O₂-free N₂-H₂-CO₂ (85:10:5) gas mixture. Stopper anaerobically, wire or seal closed and autoclave at 121C for 20 min. After autoclaving, allow to cool, open without admitting oxygen and while flushing with gas, add the following ingredients which have been filter-sterilized ahead of time:

L-Asparagine

0.25

Glucose

0.2

TPP/VFA mixture (see below)

2.0

Composition	capacity	unit
0.2% Thiamine pyrophosphate	1.5	ml
VFA solution (see below)	1.0	ml
Distilled water	8.4	ml
Mix together and filter-sterilize. Store at -20C.

Rabbit serum heat-inactivated at 56C for 1 hr

2.0

** ph : -

** memo : -

** additional actions :

Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea

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